P-252: Protective Effects of Imedeen on Oxidative Stress Induction Spermatogenic Disorders and Fertility Potential of Cyclophosphamide-Treated Male NMRI Mice.

Background: cyclophosphamide is anticancer agent that has been toxic effects on male reproductive system.The antioxidant agents in imedeen such as lycophence GS and Biomarine complex play important role in preventing the direct and indirect effects of free radicals caused by CP; therefore, the purpose of this study was to evaluate the effects of Imedeen on sperm characteristics of mature male mice that treated by CP. Materials and Methods: In this experimental study,60 mature male mice were assigned to six groups,10 mice in each. The animals allocated to control group, CP treated in 12mg/kg/day, Imedeen treated in a first dosage 111μg/kg/day, Imedeen treated in second dosage 222 μg/kg/day , CP treated and Imedeen treated in first dosage and the last group CP treated and Imedeen treated in second dosage. Sperm analysis (motion, count, morphology and viability) were evaluated at the end of experiment after 35 days. By sperm motion was assessed by computer –Assisted sperm Analysis (CASA). The date were analyzed using GB stat software. Probability values of p<0.05 and p<0.01 were considered significant. Results: The results obtained from the caudal epididymal sperm analysis revealed that treated with CP caused significant decrease in sperm count,motility,and viability,while abnormal sperms increased as compared to control.These changes were associated with significant increase in DNA damage and chromatin abnormality in the caudal epididymal spermatozoa as evidenced by Acridine Orange (AO) and Aniline Blue staining respectively. Notable administration Imedeen caused a considerable recovery in above-mentioned parameters(p<0.05). 4The results suggest that Imedeen as an antioxidant could diminish the adverse effects of cyclophosphamide in the reproductive system of male mice during cyclophosphamide administration.